p415 TEF roGFP2‐Tsa2ΔCPΔCR
(Plasmid #83239)

• Purpose: The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
• Depositing Lab: Tobias Dick
• Publication: Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. doi: 10.1038/nchembio.2067. Epub 2016 Apr 18.

Backbone

• Vector backbone: p415-TEF
• Total vector size (bp): 8000
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: roGFP2-Tsa2dCpdCr
• Species: Synthetic
• Insert Size (bp): 1500
• Promoter: TEF
• Tag / Fusion Protein:
  • roGFP2 fusion with Tsa2dCpdCr (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • p415-TEF_roGFP2-Tsa2dCpdCr.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry