Skip to main content
Addgene

pSc-His6-Sem1Sc
(Plasmid #83235)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83235 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGREG600
  • Backbone size w/o insert (bp) 7176
  • Total vector size (bp) 7476
  • Modifications to backbone
    To create the pScHis6Sem1 the GFP sequence was removed from the pGREG600 vector by Gibson assembly (Gibson et al, 2009). Then the cDHA of yeast Sem1 was sub-cloned in frame with His6 under the Gal promoter.
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418), URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sem 1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    270
  • Entrez Gene
    SEM1 (a.k.a. YDR363W-A, DSS1, HOD1)
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSc-His6-Sem1Sc was a gift from Gali Prag (Addgene plasmid # 83235 ; http://n2t.net/addgene:83235 ; RRID:Addgene_83235)

  • For your References section:

    A bacterial genetic selection system for ubiquitylation cascade discovery. Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G. Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 2016 Oct 3. 10.1038/nmeth.4003 PubMed 27694912
Related items:
Commonly requested with: