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Addgene

pCOG75-Sem1 Sc
(Plasmid #83233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-1
  • Backbone size w/o insert (bp) 4994
  • Total vector size (bp) 5264
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SEM 1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SEM1 (a.k.a. YDR363W-A, DSS1, HOD1)
  • Tag / Fusion Protein
    • His-mbp (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCOG75-Sem1 Sc was a gift from Gali Prag (Addgene plasmid # 83233 ; http://n2t.net/addgene:83233 ; RRID:Addgene_83233)

  • For your References section:

    A bacterial genetic selection system for ubiquitylation cascade discovery. Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T, Katina C, Pilzer I, Ben-Aroya S, Prag G. Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 2016 Oct 3. 10.1038/nmeth.4003 PubMed 27694912
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