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pGEX-4T2 SHP2 D61A
(Plasmid #8323)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-2
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHP2
  • Alt name
    SH-PTP2
  • Alt name
    PTPN11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2276
  • Mutation
    D61A (activated mutant)
  • GenBank ID
    L03535
  • Entrez Gene
    PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"Back-side" mutation of SHP2--activating in Xenopus and against RCM-lysozyme. Can still bind peptide through SH2 domain, further activated in vitro by peptide engagement.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T2 SHP2 D61A was a gift from Ben Neel (Addgene plasmid # 8323 ; http://n2t.net/addgene:8323 ; RRID:Addgene_8323)

  • For your References section:

    Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps. O'Reilly AM, Pluskey S, Shoelson SE, Neel BG. Mol Cell Biol 2000 Jan;20(1):299-311. 10.1128/MCB.20.1.299-311.2000 PubMed 10594032
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