pGrDL_SP
(Plasmid
#83204)
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Purposereceives miRNA target sequences for testing using the dual luciferase assay system
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGreen_dualluc_3'UTR_sensor
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Backbone manufacturerMichael Axtell
- Backbone size w/o insert (bp) 7070
- Total vector size (bp) 7086
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Modifications to backboneRemoved existing SalI site and inserted SalI/PstI cloning sites 3' of firefly LUC ORF
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSalI/PstI cloning sites
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Insert Size (bp)16
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byyes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGrDL_SP was a gift from Peer Schenk (Addgene plasmid # 83204 ; http://n2t.net/addgene:83204 ; RRID:Addgene_83204) -
For your References section:
An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences. Moyle RL, Carvalhais LC, Pretorius LS, Nowak E, Subramaniam G, Dalton-Morgan J, Schenk PM. Front Plant Sci. 2017 Sep 20;8:1631. doi: 10.3389/fpls.2017.01631. eCollection 2017. 10.3389/fpls.2017.01631 PubMed 28979287
Map uploaded by the depositor.
