CAPZB2-TwinStrepII
(Plasmid
#83194)
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PurposeExpression of tagged protein in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83194 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRP[Exp]-CMV
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Backbone manufacturerVector Builder
- Backbone size w/o insert (bp) 3855
- Total vector size (bp) 4671
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCAPZB2
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SpeciesM. musculus (mouse)
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Insert Size (bp)816
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GenBank IDNM_009798.4
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Entrez GeneCapzb (a.k.a. 1700120C01Rik, AI325129, CPB, CPB1, CPB2, CPbeat2, CPbet, CPbeta1, CPbeta2, Cap, Cappb1)
- Promoter CMV
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Tag
/ Fusion Protein
- Twin Strep Tag II (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nar1 (not destroyed)
- 3′ cloning site Bgl2 (not destroyed)
- 5′ sequencing primer None
- 3′ sequencing primer None (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAPZB2-TwinStrepII was a gift from Peter Barr-Gillespie (Addgene plasmid # 83194 ; http://n2t.net/addgene:83194 ; RRID:Addgene_83194)