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Addgene

CACNA2D1-TwinStrepII
(Plasmid #83192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRP[Exp]-CMV
  • Backbone manufacturer
    Vector Builder
  • Backbone size w/o insert (bp) 3855
  • Total vector size (bp) 7128
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CACNA2D1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3273
  • GenBank ID
    NM_012919.3
  • Entrez Gene
    Cacna2d1 (a.k.a. CCHLA2, Cacna2, DHSCCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • Twin Strep Tag II (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nar1 (not destroyed)
  • 3′ cloning site Bgl2 (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer None
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CACNA2D1-TwinStrepII was a gift from Peter Barr-Gillespie (Addgene plasmid # 83192 ; http://n2t.net/addgene:83192 ; RRID:Addgene_83192)
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