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Addgene

Hs.NRAS G12D
(Plasmid #83176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83176 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonor-255
  • Backbone size w/o insert (bp) 2693
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    591
  • Mutation
    G12D
  • GenBank ID
    NM_002524.4
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
  • 3′ sequencing primer attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hs.NRAS G12D was a gift from Dominic Esposito (Addgene plasmid # 83176 ; http://n2t.net/addgene:83176 ; RRID:Addgene_83176)
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