pmNFL
(Plasmid #83127)

• Purpose: Mouse neurofilament protein L cDNA mammalian expression construct
• Depositing Lab: Anthony Brown
• Publication: Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309.

Backbone

• Vector backbone: pEGFP-C1 with EGFP sequence removed
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4242
• Total vector size (bp): 5874
• Modifications to backbone: EGFP sequence removed using Age I and BspE I
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse NFL
• Alt name: mNFL
• Alt name: neurofilament protein L
• Species: M. musculus (mouse)
• Insert Size (bp): 1632
• GenBank ID: DQ201635
• Entrez Gene: Nefl (a.k.a. CMT2E, NF-L, NF68, Nfl)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid first described in Yan et al. (2007), "The polypeptide composition of moving and stationary neurofilaments in cultured sympathetic neurons", Cell Motility and the Cytoskeleton 64:299–309.

Mouse NFL cDNA sequence deposited in Genbank (Accession number DQ201635).