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Addgene

pcdna3.1 hciap1
(Plasmid #8311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cIAP1
  • Alt name
    BIRC2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Entrez Gene
    BIRC2 (a.k.a. API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1)
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3.1 hciap1 was a gift from Jon Ashwell (Addgene plasmid # 8311 ; http://n2t.net/addgene:8311 ; RRID:Addgene_8311)

  • For your References section:

    TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li X, Yang Y, Ashwell JD. Nature 2002 Mar 21;416(6878):345-7. 10.1038/416345a PubMed 11907583
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