pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro
(Plasmid #83107)

• Purpose: 3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
• Depositing Lab: Andrew Brooks
• Publication: Brooks lab unpublished viral plasmids (unpublished)

Backbone

• Vector backbone: pLenti PGK Puro DEST (w529-2)
• Backbone manufacturer: Eric Campeau - Addgene plasmid #19068
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: c-Myc
• Species: H. sapiens (human)
• Mutation: p64 isoform
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Promoter: hPGK
• Tag / Fusion Protein:
  • 2A self cleaving peptide (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GTGTTCCGCATTCTGCAAG

Gene/Insert 2

• Gene/Insert name: CDK4
• Species: H. sapiens (human)
• Mutation: R24C
• Entrez Gene: CDK4 (a.k.a. CMM3, PSK-J3)
• Promoter: hPGK (single transcript)

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Gene/Insert 3

• Gene/Insert name: eGFP
• Species: jellyfish
• Promoter: hPGK (single transcript)

Cloning Information for Gene/Insert 3

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-PGK-puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct contains the p64 isoform of c-Myc.