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Addgene

pLX304 PCSK5 (A270T)-V5 blast
(Plasmid #83102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Backbone manufacturer
    Addgene #25890
  • Backbone size w/o insert (bp) 9377
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PCSK5
  • Alt name
    PC5A
  • Alt name
    PC5
  • Alt name
    SPC6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2742
  • Mutation
    Changed Alanine 270 to Threonine
  • GenBank ID
  • Entrez Gene
    PCSK5 (a.k.a. PC5, PC6, PC6A, SPC6)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thermo Open Biosystems Human ORFeome version 5.1 #7239

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid for stable expression of human PC5A (PCSK5) mutant A270T with C-terminal V5 tag.

Wild-type PCSK5 sequence was mutated with QuikChange Site Directed Mutagenesis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304 PCSK5 (A270T)-V5 blast was a gift from Kevin Janes (Addgene plasmid # 83102 ; http://n2t.net/addgene:83102 ; RRID:Addgene_83102)

  • For your References section:

    Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA. Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027. 10.1016/j.devcel.2017.10.027 PubMed 29161592
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