pLX302 ID2-V5 puro
(Plasmid #83096)

• Purpose: Lentiviral vector for constitutive expression of human ID2 with C-terminal V5 tag
• Depositing Lab: Kevin Janes
• Publication: Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027.

Backbone

• Vector backbone: pLX302
• Backbone manufacturer: Addgene #25896
• Backbone size w/o insert (bp): 9573
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ID2
• Alt name: inhibitor of DNA binding 2, HLH protein
• Species: H. sapiens (human)
• Insert Size (bp): 405
• GenBank ID: NM_002166
• Entrez Gene: ID2 (a.k.a. GIG8, ID2A, ID2H, bHLHb26)
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Thermo Open Biosystems Human ORFeome version 5.1 #1362

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid for stable expression of human ID2 with C-terminal V5 tag.