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Addgene

pEN_TT 3xFLAG SMAD4
(Plasmid #83094)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83094 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN_TTmiRc2
  • Backbone manufacturer
    Addgene #83274
  • Backbone size w/o insert (bp) 4422
  • Modifications to backbone
    3xFLAG cloned in frame before restriction sites
  • Vector type
    entry vector for gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    DB3.1 (ccdB survival)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SMAD4
  • Alt name
    DPC4
  • Alt name
    MADH4
  • Alt name
    SMAD family member 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1659
  • GenBank ID
    NM_005359
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
  • Promoter TRE tight promoter
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pCEP-fwd
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR cloned from Thermo Open Biosystems Human ORFeome version 5.1 #4687

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gateway entry vector for expression of 3xFLAG SMAD4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT 3xFLAG SMAD4 was a gift from Kevin Janes (Addgene plasmid # 83094 ; http://n2t.net/addgene:83094 ; RRID:Addgene_83094)

  • For your References section:

    Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA. Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 10.1016/j.devcel.2017.10.027. 10.1016/j.devcel.2017.10.027 PubMed 29161592
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