pBbE8k-fcs
(Plasmid #83068)

• Purpose: Expresses Fcs under pBAD regulation
• Depositing Lab: Matthew Chang
• Publication: Lo et al Cell Syst. 2016 Aug;3(2):133-43. doi: 10.1016/j.cels.2016.07.012.

Backbone

• Vector backbone: pBbE8k
• Backbone manufacturer: Jay Keasling's lab
• Backbone size w/o insert (bp): 3281
• Total vector size (bp): 5165
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: fcs
• Alt name: feruloyl-CoA ligase
• Species: Pseudomonas putida KT2440
• Insert Size (bp): 1884
• Promoter: pBAD

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: Nil
• 3′ sequencing primer: Nil

Resource Information

• Supplemental Documents:
  • pBbE8k-fcs sequence (annotated)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry