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Addgene

pBbE8k-PP3359-6xHis
(Plasmid #83067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBbE8k
  • Backbone manufacturer
    Jay Keasling's lab
  • Backbone size w/o insert (bp) 3281
  • Total vector size (bp) 3903
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP3359
  • Species
    Pseudomonas putida KT2440
  • Insert Size (bp)
    468
  • Promoter pBAD
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Nil
  • 3′ sequencing primer Nil
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBbE8k-PP3359-6xHis was a gift from Matthew Chang (Addgene plasmid # 83067 ; http://n2t.net/addgene:83067 ; RRID:Addgene_83067)

  • For your References section:

    A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production. Lo TM, Chng SH, Teo WS, Cho HS, Chang MW. Cell Syst. 2016 Aug;3(2):133-43. doi: 10.1016/j.cels.2016.07.012. 10.1016/j.cels.2016.07.012 PubMed 27559924
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