p2CT-Cdi
(Plasmid
#83044)
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PurposeExpresses Corynebacterium diphtheriae Cas9 (CdiCas9)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePET
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdiCas9
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Tag
/ Fusion Protein
- His-MBP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2CT-Cdi was a gift from Jennifer Doudna (Addgene plasmid # 83044 ; http://n2t.net/addgene:83044 ; RRID:Addgene_83044) -
For your References section:
Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes. Ma E, Harrington LB, O'Connell MR, Zhou K, Doudna JA. Mol Cell. 2015 Nov 5;60(3):398-407. doi: 10.1016/j.molcel.2015.10.030. 10.1016/j.molcel.2015.10.030 PubMed 26545076