pCAG-tdTomato
(Plasmid #83029)

• Purpose: expresses tdTomato fluorescent protein under the CAG promoter
• Depositing Lab: Angelique Bordey
• Publication: Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371/journal.pone.0038174. Epub 2012 May 31.

Backbone

• Vector backbone: pCAG-CFP
• Backbone manufacturer: Cepko Lab
• Backbone size w/o insert (bp): 5551
• Total vector size (bp): 6261
• Modifications to backbone: CFP removed using AgeI and NotI
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Insert Size (bp): 1441
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The tdTomato insert was subcloned from pCMV-tdTomato (Clontech).
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCAG-CFP was used for the backbone. CFP was excised with AgeI and NotI, and tdTomato was inserted using the same restriction sites. These plasmids were also used in the following publication "Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335."