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pDONR223_CASP8_WT_V5
(Plasmid #82999)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82999 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR223
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CASP8
  • Species
    H. sapiens (human)
  • Mutation
    AA 184_191delERSSSLEGinsGEELCGVMTISD; D194R; 196_201delFSNDFGinsQDSES; S203T; 205_208delPNEKinsDKVY; 210_212delTSGinsMKSKPRGYCL; 214_216delLSDinsINN; 218_221delQQSQinsN; C223A; 225_226delSTinsAREKVPKLHSIRDRN; 228_234delESAQTSQinsTHLDAGS; 235_236 ins23
  • GenBank ID
    NM_033358
  • Entrez Gene
    CASP8 (a.k.a. ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5)
  • Promoter None

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Open state: NO stop codon immediately follows insert

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR223_CASP8_WT_V5 was a gift from Jesse Boehm & Matthew Meyerson & David Root (Addgene plasmid # 82999 ; http://n2t.net/addgene:82999 ; RRID:Addgene_82999)

  • For your References section:

    High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS. Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28. 10.1016/j.ccell.2016.06.022 PubMed 27478040
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