pLX304-CAR-V5
(Plasmid
#82723)
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PurposeExpresses V5 tagged coxsackievirus and adenovirus receptor (CAR).
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX304
- Backbone size w/o insert (bp) 9377
- Total vector size (bp) 10475
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecoxsackievirus and adenovirus receptor
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Alt nameCAR
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SpeciesH. sapiens (human)
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Insert Size (bp)1098
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GenBank IDNM_001338.4
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Entrez GeneCXADR (a.k.a. CAR, CAR4/6, HCAR)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX304-CAR-V5 was a gift from Kevin Janes (Addgene plasmid # 82723 ; http://n2t.net/addgene:82723 ; RRID:Addgene_82723) -
For your References section:
Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA. Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7. 10.1074/mcp.O116.063487 PubMed 28174228