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Addgene

pGEX-4T-1 3xFlag-MAPKAPK2
(Plasmid #82720)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82720 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 5053
  • Total vector size (bp) 6256
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use strain BL21 for protein expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAPKAPK2
  • Alt name
    mitogen-activated protein kinase-activated protein kinase 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1203
  • GenBank ID
    NM_032960.3
  • Entrez Gene
    MAPKAPK2 (a.k.a. MAPKAP-K2, MK-2, MK2)
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1 3xFlag-MAPKAPK2 was a gift from Kevin Janes (Addgene plasmid # 82720 ; http://n2t.net/addgene:82720 ; RRID:Addgene_82720)

  • For your References section:

    Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA. Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7. 10.1074/mcp.O116.063487 PubMed 28174228
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