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pCDFDuet-MKK6-EE p38alpha
(Plasmid #82719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 4864
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use strain BL21 for protein expression
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    MKK6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1005
  • Mutation
    Ser207 to Glu, Thr211 to Glu
  • GenBank ID
    NM_002758
  • Entrez Gene
    MAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    p38alpha
  • Alt name
    Mapk14
  • Alt name
    mitogen-activated protein kinase 14
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1083
  • GenBank ID
    NM_011951
  • Entrez Gene
    Mapk14 (a.k.a. CSBP2, Crk1, Csbp1, Mxi2, PRKM14, PRKM15, p38, p38-alpha, p38MAPK, p38a, p38alpha)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 Terminator Primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDFDuet-MKK6-EE p38alpha was a gift from Kevin Janes (Addgene plasmid # 82719 ; http://n2t.net/addgene:82719 ; RRID:Addgene_82719)

  • For your References section:

    Profiling Subcellular Protein Phosphatase Responses to Coxsackievirus B3 Infection of Cardiomyocytes. Shah M, Smolko CM, Kinicki S, Chapman ZD, Brautigan DL, Janes KA. Mol Cell Proteomics. 2017 Apr;16(4 suppl 1):S244-S262. doi: 10.1074/mcp.O116.063487. Epub 2017 Feb 7. 10.1074/mcp.O116.063487 PubMed 28174228
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