pBiex-1 Myosin VI HMM_SNAP
(Plasmid #82716)

• Purpose: Expresses Myosin VI HMM with a C-terminal SNAP tag.
• Depositing Lab: Sivaraj Sivaramakrishnan
• Publication: Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700. doi: 10.1038/nnano.2015.132. Epub 2015 Jul 6.

Backbone

• Vector backbone: pBiex1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5475
• Total vector size (bp): 8950
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myosin VI
• Alt name: MYO6
• Species: Sus scrofa
• Insert Size (bp): 3699
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • SNAP tag (C terminal on insert)
  • FLAG purification tag (C terminal on insert)
  • 6xHis Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 Foward
• 3′ sequencing primer: T7 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry