pBiex-1 GFPNanobody_SNAP
(Plasmid #82711)

• Purpose: Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.
• Depositing Lab: Sivaraj Sivaramakrishnan
• Publication: Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/pro.3020.

Backbone

• Vector backbone: pBiex1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5475
• Total vector size (bp): 6241
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP Nanobody
• Species: Synthetic
• Insert Size (bp): 993
• Promoter: T7 Promoter
• Tags / Fusion Proteins:
  • 6xHis Purification tag (C terminal on insert)
  • FLAG purification tag (C terminal on insert)
  • SNAP Tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 Foward
• 3′ sequencing primer: T7 Reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry