pBiex1-SYNAC
(Plasmid #82710)

• Purpose: Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator
• Depositing Lab: Sivaraj Sivaramakrishnan
• Publication: Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. doi: 10.1124/mol.115.101626. Epub 2016 Jan 22.

Backbone

• Vector backbone: pBiex1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5475
• Total vector size (bp): 9699
• Vector type: Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SYNAC
• Alt name: ADCY5
• Alt name: ADCY2
• Species: H. sapiens (human)
• Insert Size (bp): 4444
• Entrez Gene: ADCY2 (a.k.a. AC2, HBAC2)
• Entrez Gene: ADCY5 (a.k.a. AC5, DSKOD, FDFM)
• Promoter: T7
• Tags / Fusion Proteins:
  • mCerulean (N terminal on insert)
  • mCitrine (C terminal on insert)
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 FWD
• 3′ sequencing primer: T7 REV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry