p230_LTJ_4kbCD90.1Template
(Plasmid #82669)

• Purpose: 4kb HDR template for murine CD90.1
• Depositing Lab: Lukas Jeker
• Publication: Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: Genscript
• Backbone size w/o insert (bp): 2710
• Vector type: HDR template for gene editing

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 4kb genomic DNA sequence from murine CD90.1
• Species: M. musculus (mouse)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13pUC-fwd
• 3′ sequencing primer: M13pUC-rev

Resource Information

• Supplemental Documents:
  • 82669.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This HDR template can be used in conjunction with sgRNA CD90.2 (Addgene ID 82670 or Addgene ID 82671). In the template we introduced a PAM mutation. These plasmids were previously associated with the following preprint: Highly efficient and versatile plasmid-based gene editing in primary T cells bioRxiv. 13 January 2018. doi:10.1101/247544