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Addgene

Stx3-Dendra2
(Plasmid #82637)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JPA5
  • Backbone manufacturer
    Dr. John Adelman
  • Backbone size w/o insert (bp) 5410
  • Total vector size (bp) 6277
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Syntaxin-3
  • Alt name
    Stx3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    867
  • Entrez Gene
    Stx3 (a.k.a. Stx3a)
  • Promoter HCMV
  • Tag / Fusion Protein
    • Dendra2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GGTGACACTATAGAATAACATCCACTTTGC
  • 3′ sequencing primer GAGTTTGGACAAACCACAACTAGAATGCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stx3-Dendra2 was a gift from Janis Lochner (Addgene plasmid # 82637 ; http://n2t.net/addgene:82637 ; RRID:Addgene_82637)
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