tPA-Dendra2
(Plasmid #82636)

• Purpose: Expresses fluorescent hybrid of tPA for PALM imaging
• Depositing Lab: Janis Lochner
• Publication: Scalettar et al J Vis Exp. 2014 Jul 2;(89). doi: 10.3791/51394.

Backbone

• Vector backbone: JPA5
• Backbone manufacturer: Dr. John Adelman
• Backbone size w/o insert (bp): 5410
• Total vector size (bp): 7087
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Tissue plasminogen activator
• Alt name: tPA
• Species: R. norvegicus (rat)
• Insert Size (bp): 1677
• Promoter: HCMV
• Tag / Fusion Protein:
  • Dendra2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GGTGACACTATAGAATAACATCCACTTTGC
• 3′ sequencing primer: GAGTTTGGACAAACCACAACTAGAATGCAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that, compared to the Entrez Genbank reference for rat tPA, this plasmid has a E380K amino acid residue substitution.