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Addgene

pLR5-U6-sgRNA-EF
(Plasmid #82635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB-LR5
  • Vector type
    Mammalian Expression, CRISPR ; piggyBac vector
  • Promoter U6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLR5-U6-sgRNA-EF was a gift from Hiroshi Ochiai (Addgene plasmid # 82635 ; http://n2t.net/addgene:82635 ; RRID:Addgene_82635)

  • For your References section:

    Role of dynamic nuclear deformation on genomic architecture reorganization. Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto T, Ochiai H. PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 10.1371/journal.pcbi.1007289. eCollection 2019 Sep. 10.1371/journal.pcbi.1007289 PubMed 31509522
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