pLR5-CAG-NLS-tdiRFP670-2A-LBR
(Plasmid
#82620)
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PurposeExpresses NLS-tdiRFP-2A-LBR in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPB-LR5
- Total vector size (bp) 11441
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Vector typeMammalian Expression ; piggyBac vector
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNLS-tdiRFP670-LBR
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Alt nameNLS-tandem iRFP670 fused with mouse lamin B receptor (Lbr)
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)3921
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GenBank IDXM_006497071 XM_006497071
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Entrez GeneLbr (a.k.a. AI505894, C14SR, ic)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLR5-CAG-NLS-tdiRFP670-2A-LBR was a gift from Hiroshi Ochiai (Addgene plasmid # 82620 ; http://n2t.net/addgene:82620 ; RRID:Addgene_82620) -
For your References section:
Role of dynamic nuclear deformation on genomic architecture reorganization. Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto T, Ochiai H. PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 10.1371/journal.pcbi.1007289. eCollection 2019 Sep. 10.1371/journal.pcbi.1007289 PubMed 31509522