pBSKΔB-CBh-Cas9n-pA
(Plasmid
#82617)
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PurposeExpresses Cas9 nickase in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 82617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSK
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9n
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SpeciesStreptococcus pyogenes
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Insert Size (bp)4200
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSKΔB-CBh-Cas9n-pA was a gift from Hiroshi Ochiai (Addgene plasmid # 82617 ; http://n2t.net/addgene:82617 ; RRID:Addgene_82617) -
For your References section:
Role of dynamic nuclear deformation on genomic architecture reorganization. Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto T, Ochiai H. PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 10.1371/journal.pcbi.1007289. eCollection 2019 Sep. 10.1371/journal.pcbi.1007289 PubMed 31509522
Map uploaded by the depositor.
