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Addgene

p5E-Ui4-eSIBR
(Plasmid #82586)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82586 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR P4-P1R
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 4239

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human ubiquitin C promoter followed by intronically expressed eSIBR cassette
  • Insert Size (bp)
    1915
  • Promoter None

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13R
  • 3′ sequencing primer L4440 (AGCGAGTCAGTGAGCGAG)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-Ui4-eSIBR was a gift from Kryn Stankunas (Addgene plasmid # 82586 ; http://n2t.net/addgene:82586 ; RRID:Addgene_82586)

  • For your References section:

    A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P. PLoS One. 2016 Aug 8;11(8):e0159277. doi: 10.1371/journal.pone.0159277. eCollection 2016. PONE-D-16-19476 [pii] PubMed 27500400
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