pCDNA3.1-FLAG-hSF3B1-K700E
(Plasmid #82577)

• Purpose: Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)
• Depositing Lab: Manoj Pillai
• Publication: Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.2016.279.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 9391
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human SF3B1-K700E
• Alt name: splicing factor 3b subunit 1-K700E
• Species: H. sapiens (human)
• Insert Size (bp): 3963
• Mutation: K700E
• Entrez Gene: SF3B1 (a.k.a. Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG peptide (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry