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Addgene

pEMS1944
(Plasmid #82566)

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Item Catalog # Description Quantity Price (USD)
Plasmid 82566 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJ248
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple267
  • Alt name
    UGT8 MiniPromoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3014

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer rrnB-T1-term-F (5'-ACGAAAGGCTCAGTCGAAAG-3')
  • 3′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid commercially synthesized by DNA2.0

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1944 was a gift from Elizabeth Simpson (Addgene plasmid # 82566 ; http://n2t.net/addgene:82566 ; RRID:Addgene_82566)

  • For your References section:

    New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914
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