pEMS1941
(Plasmid
#82564)
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PurposeHigh copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 82564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepJ248
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Vector typePleiades Promoter Project [sic, Pleaides Plieades]
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePle264
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Alt nameNR2E1 MiniPromoter
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SpeciesH. sapiens (human)
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Insert Size (bp)3026
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer rrnB-T1-term-F (5'-ACGAAAGGCTCAGTCGAAAG-3')
- 3′ sequencing primer M13-F20 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmid commercially synthesized by DNA2.0
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS1941 was a gift from Elizabeth Simpson (Addgene plasmid # 82564 ; http://n2t.net/addgene:82564 ; RRID:Addgene_82564) -
For your References section:
Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420
Map uploaded by the depositor.
