pEMS1938
(Plasmid #82563)

• Purpose: High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.
• Depositing Lab: Elizabeth Simpson
• Publication: Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10.1016/j.exer.2019.107728.

Backbone

• Vector backbone: pJ248
• Vector type: Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ple261
• Alt name: CLDN5 MiniPromoter
• Species: H. sapiens (human)
• Insert Size (bp): 2963

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: rrnB-T1-term-F (5'-ACGAAAGGCTCAGTCGAAAG-3')
• 3′ sequencing primer: M13-F20

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• A portion of this plasmid was derived from a plasmid made by: Plasmid commercially synthesized by DNA2.0

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.

Please see the following publication for more information:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye.
de Leeuw et al. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. PMID 27164903