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Addgene

pEMS1920
(Plasmid #82562)

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Item Catalog # Description Quantity Price (USD)
Plasmid 82562 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pJ348
  • Vector type
    Pleiades Promoter Project [sic, Pleaides Plieades]

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ple253
  • Alt name
    PITX3 MiniPromoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2484

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer rrnB-T1-term-F (5'-ACGAAAGGCTCAGTCGAAAG-3')
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid commercially synthesized by DNA2.0

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEMS1920 was a gift from Elizabeth Simpson (Addgene plasmid # 82562 ; http://n2t.net/addgene:82562 ; RRID:Addgene_82562)

  • For your References section:

    Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain; Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM. Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 10.1534/genetics.119.301984. 10.1534/genetics.119.301984 PubMed 30765420
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