TET1CD-CRY2-EGFP
(Plasmid #82555)

• Purpose: Encodes TET1 under LITE2.0 system
• Depositing Lab: Joseph Irudayaraj
• Publication: Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.1038/srep42047.

Backbone

• Vector backbone: pAAV_EF1a_WPRE_hGHpA
• Backbone manufacturer: Zhang lab
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: TET1
• Species: H. sapiens (human)
• Entrez Gene: TET1 (a.k.a. CXXC6, LCX, bA119F7.1)
• Promoter: EF-1alpha

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Acc651 (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: CRY2
• Species: A. thaliana (mustard weed)
• Mutation: CRY2PHR (N-terminal photolyase homology region domain of CRY2 only)
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Tags / Fusion Proteins:
  • NLS(alpha-imp) (N terminal on backbone)
  • NLS-VP64 (C terminal on backbone)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: EGFP-N

Gene/Insert 3

• Gene/Insert name: EGFP
• Species: jellyfish
• Tag / Fusion Protein:
  • 2A (N terminal on backbone)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The insert (TET1CD) was incorporated into the ‘pAAV_EF1α_WPRE_hGHpA’ backbone of mammalian expression vector (plasmid #47457, from Zhang F, Addgene).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry