pCAG-Venus-MDGA1
(Plasmid
#82543)
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PurposeOverexpression vector for Venus-MDGA1 in neurons
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 82543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAG
- Backbone size w/o insert (bp) 5125
- Total vector size (bp) 7951
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDGA1
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SpeciesH. sapiens (human)
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Insert Size (bp)4192
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Entrez GeneMDGA1 (a.k.a. GPIM, MAMDC3)
- Promoter chicken beta actin
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Tag
/ Fusion Protein
- Venus fluorescent protein (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGCCGCCGTCCCCTTCTCCATCTC
- 3′ sequencing primer CACAGGAAACAGCTATGACCATGATTAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-Venus-MDGA1 was a gift from Alice Ting (Addgene plasmid # 82543 ; http://n2t.net/addgene:82543 ; RRID:Addgene_82543) -
For your References section:
Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts. Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY. Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 10.1016/j.cell.2016.07.041. 10.1016/j.cell.2016.07.041 PubMed 27565350
