pTRIPZ (M)-HT-Cbx7-ATLm
(Plasmid #82522)

• Purpose: Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
• Depositing Lab: Xiaojun Ren
• Publication: pTRIPZ-MSC1-Halotag-Cbx2 (unpublished)

Backbone

• Vector backbone: pTRIPZ (M)
• Total vector size (bp): 13868
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chromobox Homolog 7
• Alt name: Cbx7
• Species: H. sapiens (human)
• Insert Size (bp): 756
• Mutation: mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78
• GenBank ID: NM_175709
• Entrez Gene: CBX7
• Promoter: CMV
• Tag / Fusion Protein:
  • HaloTag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Mlu1 (unknown if destroyed)
• 5′ sequencing primer: CGATCAGGTCCGGGTTGTCTTCTTG
• 3′ sequencing primer: ctcacggcgagcgctgccacgtca

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry