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Addgene

pTRIPZ (M)-HT-NLS
(Plasmid #82518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIPZ (M)
  • Total vector size (bp) 13162
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclear localizaition sequence
  • Alt name
    NLS
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sbf1 (unknown if destroyed)
  • 5′ sequencing primer GCCGATCAGGTCCGGGTTGTCTTCT
  • 3′ sequencing primer cacggcgagcgctgccacgtcagac
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIPZ (M)-HT-NLS was a gift from Xiaojun Ren (Addgene plasmid # 82518 ; http://n2t.net/addgene:82518 ; RRID:Addgene_82518)
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