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Addgene

pTRIPZ (M)-H2A-HT
(Plasmid #82517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTRIPZ (M)
  • Backbone size w/o insert (bp) 13100
  • Total vector size (bp) 13530
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H2A
  • Alt name
    H2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    390
  • GenBank ID
    KJ892661
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PspX1 (unknown if destroyed)
  • 3′ cloning site Ecor1 (unknown if destroyed)
  • 5′ sequencing primer gcgtgtacggtgggaggcctatata
  • 3′ sequencing primer AAATCGGTACTGGCTTTCCATTCGA
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIPZ (M)-H2A-HT was a gift from Xiaojun Ren (Addgene plasmid # 82517 ; http://n2t.net/addgene:82517 ; RRID:Addgene_82517)
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