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Addgene

pOTTC840 - pAAV 5xATF6 secNLuc
(Plasmid #82497)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82497 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV EF1a
  • Backbone manufacturer
    Karl Deisseroth
  • Backbone size w/o insert (bp) 5637
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    secNLuc
  • Promoter 5x(ATF6 binding site) c-Fos minimal promoter

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC840 - pAAV 5xATF6 secNLuc was a gift from Brandon Harvey (Addgene plasmid # 82497 ; http://n2t.net/addgene:82497 ; RRID:Addgene_82497)
  • For your References section:

    Longitudinal monitoring of Gaussia and Nano luciferase activities to concurrently assess ER calcium homeostasis and ER stress in vivo. Wires ES, Henderson MJ, Yan X, Back S, Trychta KA, Lutrey MH, Harvey BK. PLoS One. 2017 Apr 12;12(4):e0175481. doi: 10.1371/journal.pone.0175481. eCollection 2017. 10.1371/journal.pone.0175481 PubMed 28403212