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Addgene

hGAPDH-rLuc
(Plasmid #82479)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82479 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    phRL-null
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3320
  • Total vector size (bp) 3840
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GAPDH promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    520
  • GenBank ID
  • Entrez Gene
    GAPDH (a.k.a. G3PD, GAPD, HEL-S-162eP)
  • Promoter hGAPDH

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer aaa
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hGAPDH-rLuc was a gift from Albee Messing (Addgene plasmid # 82479 ; http://n2t.net/addgene:82479 ; RRID:Addgene_82479)

  • For your References section:

    Dual transgenic reporter mice as a tool for monitoring expression of glial fibrillary acidic protein. Cho W, Hagemann TL, Johnson DA, Johnson JA, Messing A. J Neurochem. 2009 Jul;110(1):343-51. Epub 2009 May 5. 10.1111/j.1471-4159.2009.06146.x PubMed 19457099
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