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PurposemicroRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSMX DEST vector Addgene 62399
- Backbone size w/o insert (bp) 1950
- Total vector size (bp) 12672
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-mCherry and H2B-Citrine. HygroR
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SpeciesSynthetic
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Insert Size (bp)10740
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer TTACCGCCTTTGAGTGAG
- 3′ sequencing primer TTGTCTCATGAGCGGATAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miReporter-CAG was a gift from Pierre Neveu (Addgene plasmid # 82478 ; http://n2t.net/addgene:82478 ; RRID:Addgene_82478) -
For your References section:
Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. Sladitschek HL, Neveu PA. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016. PONE-D-16-09196 [pii] PubMed 27152616