miReporter-PGK
(Plasmid #82477)

• Purpose: microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.
• Depositing Lab: Pierre Neveu
• Publication: Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016.

Backbone

• Vector backbone: SMX DEST vector Addgene 62399
• Backbone size w/o insert (bp): 1950
• Total vector size (bp): 10036
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B-mCherry and H2B-Citrine. HygroR
• Species: Synthetic
• Insert Size (bp): 8100

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TTACCGCCTTTGAGTGAG
• 3′ sequencing primer: TTGTCTCATGAGCGGATAC

Resource Information

• Supplemental Documents:
  • annotated sequence
• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry