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pcDNA3/ TO myc-cCE-bio (cCE-bio)
(Plasmid #82473)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82473 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-T/O
  • Backbone size w/o insert (bp) 5476
  • Total vector size (bp) 7605
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio
  • Alt name
    mRNA capping enzyme
  • Species
    M. musculus (mouse), Synthetic
  • Entrez Gene
    Rngtt (a.k.a. AU020997, HCE, MCE1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (N terminal on insert)
    • TEV-Biotin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Ap1 (not destroyed)
  • 5′ sequencing primer CMV primer
  • 3′ sequencing primer SP6 primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/ TO myc-cCE-bio (cCE-bio) was a gift from Daniel Schoenberg (Addgene plasmid # 82473 ; http://n2t.net/addgene:82473 ; RRID:Addgene_82473)

  • For your References section:

    The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme. Mukherjee C, Bakthavachalu B, Schoenberg DR. PLoS Biol. 2014 Aug 19;12(8):e1001933. doi: 10.1371/journal.pbio.1001933. eCollection 2014 Aug. PBIOLOGY-D-14-00159 [pii] PubMed 25137142
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