pCDNA3-myc- mCE(Del 25C)
(Plasmid #82472)

• Purpose: Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal
• Depositing Lab: Daniel Schoenberg
• Publication: Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. doi: 10.1371/journal.pbio.1001933. eCollection 2014 Aug.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5383
• Total vector size (bp): 7134
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: myc-mCE-C terminal deleted
• Alt name: mRNA capping enzyme
• Species: M. musculus (mouse)
• Insert Size (bp): 1715
• Entrez Gene: Rngtt (a.k.a. AU020997, HCE, MCE1)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Apa1 (not destroyed)
• 5′ sequencing primer: CMV primer
• 3′ sequencing primer: SP6 primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry