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Addgene

pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag
(Plasmid #82469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4-T/O
  • Backbone size w/o insert (bp) 5008
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mRNA capping enzyme
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1911
  • Mutation
    changed Lysine 294 to Alanine
  • Entrez Gene
    Rngtt (a.k.a. AU020997, HCE, MCE1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site Apa1 (not destroyed)
  • 5′ sequencing primer CMV primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag was a gift from Daniel Schoenberg (Addgene plasmid # 82469 ; http://n2t.net/addgene:82469 ; RRID:Addgene_82469)

  • For your References section:

    Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA. Otsuka Y, Kedersha NL, Schoenberg DR. Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. Epub 2009 Feb 17. 10.1128/MCB.01325-08 PubMed 19223470
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