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Addgene

p.UTA.3.0 Empty
(Plasmid #82447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK™-2 Vector
  • Backbone manufacturer
    Promega
  • Backbone size (bp) 3360
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    acGFP from pAcGFP1-N1 Clontech and RFP from pLemiR Open Biosystems

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the following published protocol for details on plasmid use and data analysis: https://bio-protocol.org/e3000

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p.UTA.3.0 Empty was a gift from Jens Gruber (Addgene plasmid # 82447 ; http://n2t.net/addgene:82447 ; RRID:Addgene_82447)

  • For your References section:

    Dissecting miRNA gene repression on single cell level with an advanced fluorescent reporter system. Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M, Gruber J. Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197. 10.1038/srep45197 PubMed 28338079
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